Bioassay for the larvicidal activity for their larvicidal activity against different species of was carried out using who procedure with slight mosquitoes 12, 1921. Larvicidal activity of plant extracts against aedes aegypti 1217 caitied out using acetone 61 and petroleum ether 6080c, 61. Twenty nine of the thirty nine extracts tested 72% showed antibacterial activity against one or more bacteria. Larvicidal activity of plant extracts used alone and in. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. The mortality data were subjected to probit analysis to determine the lethal concentrations lc 50 and lc 90 to kill 50 and 90 per cent of the treated larvae of the respective. Many plant extracts and essential oils have been screened for their nematicidal activity against the plant parasitic nematodes814. The methanolic extract of caryophyllus aromaticus presented the highest antis. The focus of this research is the evaluation of the nematicidal activity of water extracts from pokeweed phytolacca americana. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. The present study aimed at evaluating the in vitro antimicrobial activity of plant allium sativum, zingiber officinale, caryophyllus aromaticus, cymbopogon citratus, mikania glomerata and psidium guajava extracts against grampositive and gramnegative bacterial strains isolated from human infections.
The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. In searching for natural products as potential antiviral agents, several medicinal plants from the south american folk medicine showed in vitro antiviral activity 24. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. Ten dif ferent plants were collected from in and around sivakasi area. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. Larvicidal activity of selected plant extracts against the dengue vector aedes aegypti mosquito pedro m.
Biochemistry graduates to have results from their final. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Antimicrobial activity, toxicity and phytochemical. Antibacterial activities of some plant extracts utilized.
The use of plant compounds to treat infection is an age. Antimicrobial activity of the extracts was determined by disc diffusion assays. It is therefore recommended that the nature and the number of the active antibacterial principles involved in each plant extract be studied in detail. The highest antibacterial activity of crude eos was observed in p.
Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. Antibacterial activity of crude extracts of selected plants. Such results were different from data reported in literature. Larvicidal activity of medicinal plant extracts against. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Antimicrobial activity of crude extracts of some plant leaves. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Quality of plant extract depends on plant material, choice of solvents and the extraction methods. Biological screening of various medicinal plant extracts. The plan of this work was to study the larvicidal activity of cassia occidentalis linn. Nov 05, 20 we performed the present study to evaluate the in vitro cytotoxic activity of four plant extracts that we gathered from north of iran, mazandaran materials and methods hela cells were treated with various concentrations of individual samples 0.
The chemical examination follows after the isolation and biological screening of the active fraction1. Nematicidal activity of plant extracts against the rootknot nematode, meloidogyne incognita article pdf available in the open natural products journal 21 october 2009 with 2,728 reads. Antibacterial activity of plant extracts of alstonia scholaris. Screening of in vitro antioxidant activity of methanolic leaf. Santos 2, 1department of biology, college of arts and sciences cebu normal university, osmena boulevard, cebu city, philippines. Culicidae youngcheol yang, i1kwon park, eunhee kim 2, hoiseon lee 3 and youngjoon ahn 2 department of advanced organic materials engineering, chonbuk national university, chonju 561756. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. Pdf larvicidal activity of plant extracts on aedes aegypti l. One such alternative tactic is the use of plant extracts. In relation to tetracycline activity, ethanol and ethyl acetate extracts showed similar or same activities while the water activity was higher than standard 100. None of the aqueous extracts showed antimicrobial activity. Larvicidal activities of three medicinal plant extracts were studied in the range of 4. While some plant extracts were comparatively more effective against all the three pathogens xanthomonas campestris, agrobacterium rhizogenes and aspergillus fumigatus.
Effects of allelochemical extracts from medicinal plants. Plant products yield extracts with antimicrobial activities. Euphorbia fischeriana steud is a perennial herbaceous plant with latex, distributed mainly in north china 15. Larvicidal activity of selected plant extracts against the.
All plant extracts showed moderate effects after 24 h of exposure. Biological screening of various medicinal plant extracts for. Antibacterial australian plants griffith university. Chemical composition and antibacterial activity of. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Pdf nematicidal activity of plant extracts against the root. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. They transmit the parasites and pathogens which cause a deadly disease like filariasis, dengue, yellow fever, malaria, japanese encephalitis, chikungunya, and so. Phytochemical investigation and biological activity of leaves. They transmit the parasites and pathogens which cause a deadly disease like filariasis, dengue, yellow fever, malaria, japanese encephalitis, chikungunya, and so forth, which are.
Cytotoxic properties of some medicinal plant extracts from. Plants are collected either randomly or by following leads supplied by local healers in geographical areas where the plants are found. The abts scavenging capacity of the extract was compared with that of bht and percentage inhibition calculated as abts radical scavenging activity % abs control. Assessment of antioxidant activity of plant extracts by. Larvicidal activity of plant extracts on aedes aegypti l article pdf available in asian pacific journal of tropical biomedicine 23. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. Fresh or dried plant materials can be used as a source for the extraction of secondary. From the stock solution, concentrations activity are methanolic stem extracts of satureja hortensis. Antimicrobial activity of single and combined extracts of. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Antimicrobial activity of some plant extracts against.
Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. The activity of crude essential oils against the three bacteria isolates was within the extremely sensitive category diameters of the inhibition zone larger than 20 mm after 24 and 48 hours of incubation table 1. Plant extracts have shown inhibitory effect on the growth of the bacteria studied, although of distinct forms. The biological activity of plant extracts might be due to various compounds, including phenolics, terpenoids, and alkaloids present against, and against. As an alternative to synthetic drugs, the consolidation of empirical knowledge with ethnopharmacological evidence of medicinal plants offers a novel platform for the development of antiviral drugs.
Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Antimicrobial activity of crude extracts from plant parts and corresponding calli of f bixa orellana l. All these plant extracts were made free from solvent by steam distillation, concentrated by evaporation and were used for the larvicidal testing by standard procedure5. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al.
The emergence of drug resistant variants of the influenza virus has led to a need to identify novel and effective antiviral agents. Studies on some plant extracts for their antimicrobial. Studies on in vitro antimicrobial activity of ethanol extracts of rauvolfia tetraphylla. Biochemistry graduates to have results from their final year. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. Phytochemical analysis of phyllanthus amarus extracts s. A laboratory exercise on determining the antioxidant activity of plant extracts jacinto et al. The results showed that extracts of mint, lemon balm and coriander among others inhibited the growth of e. The effect of various plant extracts was measured on several important oxidation indicators. Pdf cytotoxic activity of a plant extract on cancer cells. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. Antibacterial activity of plants 719 effective extracts are considered, e. Antibacterial activities of some borneo plant extracts.
Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus. Antimicrobial activity of selected medicinal plants against. Evaluation of nematicidal activity of ethanol extracts of. All these extracts present a linear variation of the inhibition power with the added concentration of extract fig. Varoni 1,3,4, fr anco fraschini 2, lia rimondini 1.
At present quick and effective management of plant dis eases and microbial contaminations is generally achieved by the use of synthetic pesticides 68. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Antioxidant and in vitro cytotoxic activity of extracts of.
Forty eight extracts from 38 medicinal plants distributed into 19 different plant families were evaluated for cytotoxic activity on mammalian cells llcmk2 cells as presented in table 1. Cytoto xic activity of a plant extract on cancer cells enrico catalano 1, paolo maestri 2, andrea cochis 1, barbara az zimonti 1, ele na m. Efficacy of plant extracts in plant disease management. Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita. Effects of allelochemical extracts from medicinal plants on. Identification of traditional medicinal plant extracts. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Determining the antioxidant property of plant extracts. Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1. Antimicrobial activity of crude extracts of three medicinal. Antioxidant activity of some algerian medicinal plants. The most active plant was torilis anthriscus especially in relation to pseudomonas glycinea. Screening of antibacterial activity of the medicinal plant.
Antimicrobial activity of aqueous and organic extracts. Pdf nematicidal activity of plant extracts against the. Evaluation of the cytotoxic activity of extracts from. Phytochemical investigation and biological activity of. In the present study, we evaluated the potential of 5 selected plants to inhibit test bacteria by agar well diffusion assay and the result is shown in table 2. Species aegopodium podagraria, daucus carota, heracleum sphondylium and pimpinella. Larvicidal efficacy of plant extracts against the malarial. Indeed, of the twenty five plant species tested only 3 species had no inhibitory activity towards any of the bacteria tested in any of their. Natural plant extracts have been tested in the laboratory against bacteria and fungi. The decrease in absorbance for each extract in intervals of time is determined, and plotted. Plant extracts 1 ml were allowed to react with 1 ml of the abts solution and the absorbance was taken at 734 nm after 7 min using the spectrophotometer.
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